Construct: shRNA TRCN0000347450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034878.2-1281s21c1
DNA Barcode:
GCCCATCGAAGTTGTCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnaic2 (432611)
Gene Description:
dynein, axonemal, intermediate chain 2
Transcript:
RefSeq NM_001034878.2 (CURRENT)
Match location:
Position 1281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATCGAAGTTGTCATTAT
Hairpin Sequence:
5'-CCGG-GCCCATCGAAGTTGTCATTAT-CTCGAG-ATAATGACAACTTCGATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATCGAAGTTGTCATTATCTCGAGATAATGACAACTTCGATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATCGAAGTTGTCATTATCTCGAGATAATGACAACTTCGATGGGC-3'

Other clones with same target sequence:

(none)