Construct: shRNA TRCN0000347499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146022.1-1172s21c1
DNA Barcode:
CAAGGTACTGCTGCGGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdfy4 (545030)
Gene Description:
WD repeat and FYVE domain containing 4
Transcript:
RefSeq NM_001146022.1 (NON-CURRENT)
Match location:
Position 1172 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGTACTGCTGCGGTATAA
Hairpin Sequence:
5'-CCGG-CAAGGTACTGCTGCGGTATAA-CTCGAG-TTATACCGCAGCAGTACCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGTACTGCTGCGGTATAACTCGAGTTATACCGCAGCAGTACCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGTACTGCTGCGGTATAACTCGAGTTATACCGCAGCAGTACCTTG-3'

Other clones with same target sequence:

(none)