Construct: shRNA TRCN0000347504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011207.2-3418s21c1
DNA Barcode:
GAGGATGAACAGGATCTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn3 (545622)
Gene Description:
protein tyrosine phosphatase, non-receptor type 3
Transcript:
RefSeq NM_011207.2 (CURRENT)
Match location:
Position 3418 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGATGAACAGGATCTATTC
Hairpin Sequence:
5'-CCGG-GAGGATGAACAGGATCTATTC-CTCGAG-GAATAGATCCTGTTCATCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGATGAACAGGATCTATTCCTCGAGGAATAGATCCTGTTCATCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGATGAACAGGATCTATTCCTCGAGGAATAGATCCTGTTCATCCTC-3'

Other clones with same target sequence:

(none)