Construct: shRNA TRCN0000347671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145198.1-274s21c1
DNA Barcode:
CAACATGGCGTCTAGTCAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1500009L16Rik (69784)
Gene Description:
RIKEN cDNA 1500009L16 gene
Transcript:
RefSeq NM_001145198.1 (CURRENT)
Match location:
Position 274 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACATGGCGTCTAGTCAAAC
Hairpin Sequence:
5'-CCGG-CAACATGGCGTCTAGTCAAAC-CTCGAG-GTTTGACTAGACGCCATGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACATGGCGTCTAGTCAAACCTCGAGGTTTGACTAGACGCCATGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACATGGCGTCTAGTCAAACCTCGAGGTTTGACTAGACGCCATGTTG-3'

Other clones with same target sequence:

(none)