Construct: shRNA TRCN0000347751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145198.1-192s21c1
DNA Barcode:
ACACAACAGAAGACTCGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1500009L16Rik (69784)
Gene Description:
RIKEN cDNA 1500009L16 gene
Transcript:
RefSeq NM_001145198.1 (CURRENT)
Match location:
Position 192 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACAACAGAAGACTCGATAA
Hairpin Sequence:
5'-CCGG-ACACAACAGAAGACTCGATAA-CTCGAG-TTATCGAGTCTTCTGTTGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACAACAGAAGACTCGATAACTCGAGTTATCGAGTCTTCTGTTGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACAACAGAAGACTCGATAACTCGAGTTATCGAGTCTTCTGTTGTGT-3'

Other clones with same target sequence:

(none)