Construct: shRNA TRCN0000347814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001162364.1-543s21c1
DNA Barcode:
GTGTGAGCAGAAATTTGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14525 (100039120)
Gene Description:
predicted gene 14525
Transcript:
RefSeq NM_001162364.1 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGAGCAGAAATTTGATAT
Hairpin Sequence:
5'-CCGG-GTGTGAGCAGAAATTTGATAT-CTCGAG-ATATCAAATTTCTGCTCACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGAGCAGAAATTTGATATCTCGAGATATCAAATTTCTGCTCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGAGCAGAAATTTGATATCTCGAGATATCAAATTTCTGCTCACAC-3'

Other clones with same target sequence:

(none)