Construct: shRNA TRCN0000347836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001160129.1-490s21c1
DNA Barcode:
CTCTTTATTTGGTGAAGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm20852 (100040786)
Gene Description:
predicted gene, 20852
Transcript:
RefSeq NM_001160129.1 (NON-CURRENT)
Match location:
Position 490 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTTATTTGGTGAAGTATG
Hairpin Sequence:
5'-CCGG-CTCTTTATTTGGTGAAGTATG-CTCGAG-CATACTTCACCAAATAAAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTTATTTGGTGAAGTATGCTCGAGCATACTTCACCAAATAAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTTATTTGGTGAAGTATGCTCGAGCATACTTCACCAAATAAAGAG-3'

Other clones with same target sequence:

(none)