Construct: shRNA TRCN0000347838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001160129.1-575s21c1
DNA Barcode:
CTTGCCTCACAAAGTAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm20852 (100040786)
Gene Description:
predicted gene, 20852
Transcript:
RefSeq NM_001160129.1 (NON-CURRENT)
Match location:
Position 575 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGCCTCACAAAGTAGATTT
Hairpin Sequence:
5'-CCGG-CTTGCCTCACAAAGTAGATTT-CTCGAG-AAATCTACTTTGTGAGGCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGCCTCACAAAGTAGATTTCTCGAGAAATCTACTTTGTGAGGCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGCCTCACAAAGTAGATTTCTCGAGAAATCTACTTTGTGAGGCAAG-3'

Other clones with same target sequence:

(none)