Construct: shRNA TRCN0000347896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001160140.1-543s21c1
DNA Barcode:
CAACAAACCCTTCTCTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm21394 (100040744)
Gene Description:
predicted gene, 21394
Transcript:
RefSeq NM_001160140.1 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAAACCCTTCTCTTTATT
Hairpin Sequence:
5'-CCGG-CAACAAACCCTTCTCTTTATT-CTCGAG-AATAAAGAGAAGGGTTTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAAACCCTTCTCTTTATTCTCGAGAATAAAGAGAAGGGTTTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAAACCCTTCTCTTTATTCTCGAGAATAAAGAGAAGGGTTTGTTG-3'

Other clones with same target sequence:

(none)