Construct: shRNA TRCN0000347912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001160131.1-382s21c1
DNA Barcode:
TCCACAGCGATGAGAGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm20854 (100040911)
Gene Description:
predicted gene, 20854
Transcript:
RefSeq NM_001160131.1 (CURRENT)
Match location:
Position 382 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCACAGCGATGAGAGGATTT
Hairpin Sequence:
5'-CCGG-TCCACAGCGATGAGAGGATTT-CTCGAG-AAATCCTCTCATCGCTGTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCACAGCGATGAGAGGATTTCTCGAGAAATCCTCTCATCGCTGTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCACAGCGATGAGAGGATTTCTCGAGAAATCCTCTCATCGCTGTGGA-3'

Other clones with same target sequence:

(none)