Construct: shRNA TRCN0000347938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001160137.1-768s21c1
DNA Barcode:
TTACAAAGTTCTAGCCAATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm20809 (100039614)
Gene Description:
predicted gene, 20809
Transcript:
RefSeq NM_001160137.1 (CURRENT)
Match location:
Position 768 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACAAAGTTCTAGCCAATTC
Hairpin Sequence:
5'-CCGG-TTACAAAGTTCTAGCCAATTC-CTCGAG-GAATTGGCTAGAACTTTGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACAAAGTTCTAGCCAATTCCTCGAGGAATTGGCTAGAACTTTGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACAAAGTTCTAGCCAATTCCTCGAGGAATTGGCTAGAACTTTGTAA-3'

Other clones with same target sequence:

(none)