Construct: shRNA TRCN0000348197

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039546.1-2397s21c1
DNA Barcode:
AGATTGGATCCGAGGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo6 (17920)
Gene Description:
myosin VI
Transcript:
RefSeq NM_001039546.1 (NON-CURRENT)
Match location:
Position 2397 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348197)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTGGATCCGAGGTTATTT
Hairpin Sequence:
5'-CCGG-AGATTGGATCCGAGGTTATTT-CTCGAG-AAATAACCTCGGATCCAATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTGGATCCGAGGTTATTTCTCGAGAAATAACCTCGGATCCAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTGGATCCGAGGTTATTTCTCGAGAAATAACCTCGGATCCAATCT-3'

Other clones with same target sequence:

(none)