Construct: shRNA TRCN0000348199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011428.3-889s21c1
DNA Barcode:
CATGCTTCTCTCATGGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snap25 (20614)
Gene Description:
synaptosomal-associated protein 25
Transcript:
RefSeq NM_011428.3 (CURRENT)
Match location:
Position 889 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGCTTCTCTCATGGTATTA
Hairpin Sequence:
5'-CCGG-CATGCTTCTCTCATGGTATTA-CTCGAG-TAATACCATGAGAGAAGCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGCTTCTCTCATGGTATTACTCGAGTAATACCATGAGAGAAGCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGCTTCTCTCATGGTATTACTCGAGTAATACCATGAGAGAAGCATG-3'

Other clones with same target sequence:

(none)