Construct: shRNA TRCN0000348246

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016921.2-648s21c1
DNA Barcode:
ATTCAGACCTGAAGGTCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcirg1 (27060)
Gene Description:
T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
Transcript:
RefSeq NM_016921.2 (NON-CURRENT)
Match location:
Position 648 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348246)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCAGACCTGAAGGTCAATT
Hairpin Sequence:
5'-CCGG-ATTCAGACCTGAAGGTCAATT-CTCGAG-AATTGACCTTCAGGTCTGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCAGACCTGAAGGTCAATTCTCGAGAATTGACCTTCAGGTCTGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCAGACCTGAAGGTCAATTCTCGAGAATTGACCTTCAGGTCTGAAT-3'

Other clones with same target sequence:

(none)