Construct: shRNA TRCN0000348267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029505.3-671s21c1
DNA Barcode:
TACCAACAACGAGGCCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap3m2 (64933)
Gene Description:
adaptor-related protein complex 3, mu 2 subunit
Transcript:
RefSeq NM_029505.3 (CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCAACAACGAGGCCTATTT
Hairpin Sequence:
5'-CCGG-TACCAACAACGAGGCCTATTT-CTCGAG-AAATAGGCCTCGTTGTTGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCAACAACGAGGCCTATTTCTCGAGAAATAGGCCTCGTTGTTGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCAACAACGAGGCCTATTTCTCGAGAAATAGGCCTCGTTGTTGGTA-3'

Other clones with same target sequence:

(none)