Construct: shRNA TRCN0000348322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173047.3-759s21c1
DNA Barcode:
CGGACAGGATTCTGCTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbr3 (109857)
Gene Description:
carbonyl reductase 3
Transcript:
RefSeq NM_173047.3 (CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACAGGATTCTGCTCAATG
Hairpin Sequence:
5'-CCGG-CGGACAGGATTCTGCTCAATG-CTCGAG-CATTGAGCAGAATCCTGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACAGGATTCTGCTCAATGCTCGAGCATTGAGCAGAATCCTGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACAGGATTCTGCTCAATGCTCGAGCATTGAGCAGAATCCTGTCCG-3'

Other clones with same target sequence:

(none)