Construct: shRNA TRCN0000348336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012015.2-1051s21c1
DNA Barcode:
GTTTGTGATCCACTGTAATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Macroh2a1 (26914)
Gene Description:
macroH2A.1 histone
Transcript:
RefSeq NM_012015.2 (CURRENT)
Match location:
Position 1051 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGTGATCCACTGTAATAG
Hairpin Sequence:
5'-CCGG-GTTTGTGATCCACTGTAATAG-CTCGAG-CTATTACAGTGGATCACAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGTGATCCACTGTAATAGCTCGAGCTATTACAGTGGATCACAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGTGATCCACTGTAATAGCTCGAGCTATTACAGTGGATCACAAAC-3'

Other clones with same target sequence:

(none)