Construct: shRNA TRCN0000348372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024290.3-695s21c1
DNA Barcode:
CGGACTAGGCACCTCTAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfrsf23 (79201)
Gene Description:
tumor necrosis factor receptor superfamily, member 23
Transcript:
RefSeq NM_024290.3 (NON-CURRENT)
Match location:
Position 695 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACTAGGCACCTCTAATAT
Hairpin Sequence:
5'-CCGG-CGGACTAGGCACCTCTAATAT-CTCGAG-ATATTAGAGGTGCCTAGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACTAGGCACCTCTAATATCTCGAGATATTAGAGGTGCCTAGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACTAGGCACCTCTAATATCTCGAGATATTAGAGGTGCCTAGTCCG-3'

Other clones with same target sequence:

(none)