Construct: shRNA TRCN0000348431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010681.3-3795s21c1
DNA Barcode:
GATATCTCGCAGAGCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lama4 (16775)
Gene Description:
laminin, alpha 4
Transcript:
RefSeq NM_010681.3 (NON-CURRENT)
Match location:
Position 3795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATATCTCGCAGAGCATATTT
Hairpin Sequence:
5'-CCGG-GATATCTCGCAGAGCATATTT-CTCGAG-AAATATGCTCTGCGAGATATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATATCTCGCAGAGCATATTTCTCGAGAAATATGCTCTGCGAGATATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATATCTCGCAGAGCATATTTCTCGAGAAATATGCTCTGCGAGATATC-3'

Other clones with same target sequence:

(none)