Construct: shRNA TRCN0000348470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144491.2-273s21c1
DNA Barcode:
TGCCTGCACTATTGTGGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dph1 (116905)
Gene Description:
diphthamide biosynthesis 1
Transcript:
RefSeq NM_144491.2 (CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTGCACTATTGTGGATAT
Hairpin Sequence:
5'-CCGG-TGCCTGCACTATTGTGGATAT-CTCGAG-ATATCCACAATAGTGCAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTGCACTATTGTGGATATCTCGAGATATCCACAATAGTGCAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTGCACTATTGTGGATATCTCGAGATATCCACAATAGTGCAGGCA-3'

Other clones with same target sequence:

(none)