Construct: shRNA TRCN0000348492
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_144731.3-995s21c1
- DNA Barcode:
- ATTTGCACTGTGCCGATTATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Galnt7 (108150)
- Gene Description:
- UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7
- Transcript:
- RefSeq NM_144731.3 (NON-CURRENT)
- Match location:
- Position 995 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000348492)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ATTTGCACTGTGCCGATTATA
- Hairpin Sequence:
-
5'-CCGG-ATTTGCACTGTGCCGATTATA-CTCGAG-TATAATCGGCACAGTGCAAAT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGATTTGCACTGTGCCGATTATACTCGAGTATAATCGGCACAGTGCAAATTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAATTTGCACTGTGCCGATTATACTCGAGTATAATCGGCACAGTGCAAAT-3'
Other clones with same target sequence:
(none)
