Construct: shRNA TRCN0000348533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207302.1-516s21c1
DNA Barcode:
TGACCATCCCAGACCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zranb1 (360216)
Gene Description:
zinc finger, RAN-binding domain containing 1
Transcript:
RefSeq NM_207302.1 (CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACCATCCCAGACCTAATAA
Hairpin Sequence:
5'-CCGG-TGACCATCCCAGACCTAATAA-CTCGAG-TTATTAGGTCTGGGATGGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACCATCCCAGACCTAATAACTCGAGTTATTAGGTCTGGGATGGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACCATCCCAGACCTAATAACTCGAGTTATTAGGTCTGGGATGGTCA-3'

Other clones with same target sequence:

(none)