Construct: shRNA TRCN0000348570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008087.2-1293s21c1
DNA Barcode:
CTCAAATGGTAGCACTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gas2 (14453)
Gene Description:
growth arrest specific 2
Transcript:
RefSeq NM_008087.2 (NON-CURRENT)
Match location:
Position 1293 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAAATGGTAGCACTATTTA
Hairpin Sequence:
5'-CCGG-CTCAAATGGTAGCACTATTTA-CTCGAG-TAAATAGTGCTACCATTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAAATGGTAGCACTATTTACTCGAGTAAATAGTGCTACCATTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAAATGGTAGCACTATTTACTCGAGTAAATAGTGCTACCATTTGAG-3'

Other clones with same target sequence:

(none)