Construct: shRNA TRCN0000348624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010578.2-2893s21c1
DNA Barcode:
GACTTGAGAAAGGATCTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itgb1 (16412)
Gene Description:
integrin beta 1 (fibronectin receptor beta)
Transcript:
RefSeq NM_010578.2 (CURRENT)
Match location:
Position 2893 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTGAGAAAGGATCTTTAT
Hairpin Sequence:
5'-CCGG-GACTTGAGAAAGGATCTTTAT-CTCGAG-ATAAAGATCCTTTCTCAAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTGAGAAAGGATCTTTATCTCGAGATAAAGATCCTTTCTCAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTGAGAAAGGATCTTTATCTCGAGATAAAGATCCTTTCTCAAGTC-3'

Other clones with same target sequence:

(none)