Construct: shRNA TRCN0000348673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053269.3-902s21c1
DNA Barcode:
GATGACAACAAAGATTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad51c (114714)
Gene Description:
RAD51 paralog C
Transcript:
RefSeq NM_053269.3 (CURRENT)
Match location:
Position 902 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGACAACAAAGATTGATAA
Hairpin Sequence:
5'-CCGG-GATGACAACAAAGATTGATAA-CTCGAG-TTATCAATCTTTGTTGTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGACAACAAAGATTGATAACTCGAGTTATCAATCTTTGTTGTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGACAACAAAGATTGATAACTCGAGTTATCAATCTTTGTTGTCATC-3'

Other clones with same target sequence:

(none)