Construct: shRNA TRCN0000348687

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145596.3-1383s21c1
DNA Barcode:
GATCATCCAGCAGGGACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gatad2a (234366)
Gene Description:
GATA zinc finger domain containing 2A
Transcript:
RefSeq NM_145596.3 (NON-CURRENT)
Match location:
Position 1383 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348687)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCATCCAGCAGGGACTTAT
Hairpin Sequence:
5'-CCGG-GATCATCCAGCAGGGACTTAT-CTCGAG-ATAAGTCCCTGCTGGATGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCATCCAGCAGGGACTTATCTCGAGATAAGTCCCTGCTGGATGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCATCCAGCAGGGACTTATCTCGAGATAAGTCCCTGCTGGATGATC-3'

Other clones with same target sequence:

(none)