Construct: shRNA TRCN0000348817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199307.2-1322s21c1
DNA Barcode:
TCATCAACAACACCGACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ece1 (230857)
Gene Description:
endothelin converting enzyme 1
Transcript:
RefSeq NM_199307.2 (CURRENT)
Match location:
Position 1322 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCAACAACACCGACAAAT
Hairpin Sequence:
5'-CCGG-TCATCAACAACACCGACAAAT-CTCGAG-ATTTGTCGGTGTTGTTGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCAACAACACCGACAAATCTCGAGATTTGTCGGTGTTGTTGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCAACAACACCGACAAATCTCGAGATTTGTCGGTGTTGTTGATGA-3'

Other clones with same target sequence:

(none)