Construct: shRNA TRCN0000348929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172684.2-1127s21c1
DNA Barcode:
ATGAGAGGAAGACGCCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rsbn1 (229675)
Gene Description:
rosbin, round spermatid basic protein 1
Transcript:
RefSeq NM_172684.2 (CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGAGGAAGACGCCTTAAA
Hairpin Sequence:
5'-CCGG-ATGAGAGGAAGACGCCTTAAA-CTCGAG-TTTAAGGCGTCTTCCTCTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGAGGAAGACGCCTTAAACTCGAGTTTAAGGCGTCTTCCTCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGAGGAAGACGCCTTAAACTCGAGTTTAAGGCGTCTTCCTCTCAT-3'

Other clones with same target sequence:

(none)