Construct: shRNA TRCN0000349088

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027030.2-649s21c1
DNA Barcode:
GCAGCTTGATGACCTGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcps (69305)
Gene Description:
decapping enzyme, scavenger
Transcript:
RefSeq NM_027030.2 (CURRENT)
Match location:
Position 649 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349088)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTTGATGACCTGTATTT
Hairpin Sequence:
5'-CCGG-GCAGCTTGATGACCTGTATTT-CTCGAG-AAATACAGGTCATCAAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTTGATGACCTGTATTTCTCGAGAAATACAGGTCATCAAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTTGATGACCTGTATTTCTCGAGAAATACAGGTCATCAAGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000126461