Construct: shRNA TRCN0000349188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025522.5-807s21c1
DNA Barcode:
CCGAGGAAGTAACTAAGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhrs7 (66375)
Gene Description:
dehydrogenase/reductase (SDR family) member 7
Transcript:
RefSeq NM_025522.5 (CURRENT)
Match location:
Position 807 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGGAAGTAACTAAGTCTA
Hairpin Sequence:
5'-CCGG-CCGAGGAAGTAACTAAGTCTA-CTCGAG-TAGACTTAGTTACTTCCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGGAAGTAACTAAGTCTACTCGAGTAGACTTAGTTACTTCCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGGAAGTAACTAAGTCTACTCGAGTAGACTTAGTTACTTCCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000041573