Construct: shRNA TRCN0000349191

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019654.2-1286s21c1
DNA Barcode:
GCACAAATACACACCTTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Socs5 (56468)
Gene Description:
suppressor of cytokine signaling 5
Transcript:
RefSeq NM_019654.2 (CURRENT)
Match location:
Position 1286 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349191)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAATACACACCTTTGAA
Hairpin Sequence:
5'-CCGG-GCACAAATACACACCTTTGAA-CTCGAG-TTCAAAGGTGTGTATTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAATACACACCTTTGAACTCGAGTTCAAAGGTGTGTATTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAATACACACCTTTGAACTCGAGTTCAAAGGTGTGTATTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000105751