Construct: shRNA TRCN0000349347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020642.3-762s21c1
DNA Barcode:
CCACTGTATGATTCTCTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKIP1 (56672)
Gene Description:
A-kinase interacting protein 1
Transcript:
RefSeq NM_020642.3 (NON-CURRENT)
Match location:
Position 762 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGTATGATTCTCTTAAT
Hairpin Sequence:
5'-CCGG-CCACTGTATGATTCTCTTAAT-CTCGAG-ATTAAGAGAATCATACAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGTATGATTCTCTTAATCTCGAGATTAAGAGAATCATACAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGTATGATTCTCTTAATCTCGAGATTAAGAGAATCATACAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000082528