Construct: shRNA TRCN0000349352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009537.3-811s21c1
DNA Barcode:
GTGGTTGAAGAGCAGATCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Yy1 (22632)
Gene Description:
YY1 transcription factor
Transcript:
RefSeq NM_009537.3 (CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTTGAAGAGCAGATCATT
Hairpin Sequence:
5'-CCGG-GTGGTTGAAGAGCAGATCATT-CTCGAG-AATGATCTGCTCTTCAACCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTTGAAGAGCAGATCATTCTCGAGAATGATCTGCTCTTCAACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTTGAAGAGCAGATCATTCTCGAGAATGATCTGCTCTTCAACCAC-3'

Other clones with same target sequence:

  1. TRCN0000054556