Construct: shRNA TRCN0000349560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001229.2-1292s21c1
DNA Barcode:
GATGCCTGGTTGCTTTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CASP9 (842)
Gene Description:
caspase 9
Transcript:
RefSeq NM_001229.2 (NON-CURRENT)
Match location:
Position 1292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCCTGGTTGCTTTAATTT
Hairpin Sequence:
5'-CCGG-GATGCCTGGTTGCTTTAATTT-CTCGAG-AAATTAAAGCAACCAGGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCCTGGTTGCTTTAATTTCTCGAGAAATTAAAGCAACCAGGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCCTGGTTGCTTTAATTTCTCGAGAAATTAAAGCAACCAGGCATC-3'

Other clones with same target sequence:

  1. TRCN0000003583