Construct: shRNA TRCN0000349627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003470.2-3665s21c1
DNA Barcode:
TGTATCTATTGACTGCCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP7 (7874)
Gene Description:
ubiquitin specific peptidase 7
Transcript:
RefSeq NM_003470.2 (NON-CURRENT)
Match location:
Position 3665 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTATCTATTGACTGCCCTTT
Hairpin Sequence:
5'-CCGG-TGTATCTATTGACTGCCCTTT-CTCGAG-AAAGGGCAGTCAATAGATACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTATCTATTGACTGCCCTTTCTCGAGAAAGGGCAGTCAATAGATACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTATCTATTGACTGCCCTTTCTCGAGAAAGGGCAGTCAATAGATACA-3'

Other clones with same target sequence:

  1. TRCN0000004059