Construct: shRNA TRCN0000349629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005066.2-1483s21c1
DNA Barcode:
GCCCAGAAGAATCCAATGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SFPQ (6421)
Gene Description:
splicing factor proline and glutamine rich
Transcript:
RefSeq NM_005066.2 (NON-CURRENT)
Match location:
Position 1483 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGAAGAATCCAATGTAT
Hairpin Sequence:
5'-CCGG-GCCCAGAAGAATCCAATGTAT-CTCGAG-ATACATTGGATTCTTCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGAAGAATCCAATGTATCTCGAGATACATTGGATTCTTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGAAGAATCCAATGTATCTCGAGATACATTGGATTCTTCTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000001082