Construct: shRNA TRCN0000349667

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016145.3-347s21c1
DNA Barcode:
GCCGGACTACATGAACCTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR83OS (51398)
Gene Description:
WD repeat domain 83 opposite strand
Transcript:
RefSeq NM_016145.3 (NON-CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349667)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGACTACATGAACCTGCT
Hairpin Sequence:
5'-CCGG-GCCGGACTACATGAACCTGCT-CTCGAG-AGCAGGTTCATGTAGTCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGACTACATGAACCTGCTCTCGAGAGCAGGTTCATGTAGTCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGACTACATGAACCTGCTCTCGAGAGCAGGTTCATGTAGTCCGGC-3'

Other clones with same target sequence:

  1. TRCN0000122866