Construct: shRNA TRCN0000349668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013436.3-1416s21c1
DNA Barcode:
GCACAGAGAAAGACGCAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCKAP1 (10787)
Gene Description:
NCK associated protein 1
Transcript:
RefSeq NM_013436.3 (NON-CURRENT)
Match location:
Position 1416 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGAGAAAGACGCAAGTT
Hairpin Sequence:
5'-CCGG-GCACAGAGAAAGACGCAAGTT-CTCGAG-AACTTGCGTCTTTCTCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGAGAAAGACGCAAGTTCTCGAGAACTTGCGTCTTTCTCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGAGAAAGACGCAAGTTCTCGAGAACTTGCGTCTTTCTCTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000154905