Construct: shRNA TRCN0000349717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182487.2-4523s21c1
DNA Barcode:
CCGCCAAACAAACATTCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OLFML2A (169611)
Gene Description:
olfactomedin like 2A
Transcript:
RefSeq NM_182487.2 (NON-CURRENT)
Match location:
Position 4523 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCCAAACAAACATTCACTA
Hairpin Sequence:
5'-CCGG-CCGCCAAACAAACATTCACTA-CTCGAG-TAGTGAATGTTTGTTTGGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCCAAACAAACATTCACTACTCGAGTAGTGAATGTTTGTTTGGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCCAAACAAACATTCACTACTCGAGTAGTGAATGTTTGTTTGGCGG-3'

Other clones with same target sequence:

  1. TRCN0000203930