Construct: shRNA TRCN0000349743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004393.2-2554s21c1
DNA Barcode:
TTGTCGGCACCTACAGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAG1 (1605)
Gene Description:
dystroglycan 1
Transcript:
RefSeq NM_004393.2 (NON-CURRENT)
Match location:
Position 2554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTCGGCACCTACAGTTTAT
Hairpin Sequence:
5'-CCGG-TTGTCGGCACCTACAGTTTAT-CTCGAG-ATAAACTGTAGGTGCCGACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTCGGCACCTACAGTTTATCTCGAGATAAACTGTAGGTGCCGACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTCGGCACCTACAGTTTATCTCGAGATAAACTGTAGGTGCCGACAA-3'

Other clones with same target sequence:

(none)