Construct: shRNA TRCN0000349762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009572.3-3499s21c1
DNA Barcode:
ATGTATATAGAGCGCTAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zhx1 (22770)
Gene Description:
zinc fingers and homeoboxes 1
Transcript:
RefSeq NM_009572.3 (NON-CURRENT)
Match location:
Position 3499 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTATATAGAGCGCTAATTA
Hairpin Sequence:
5'-CCGG-ATGTATATAGAGCGCTAATTA-CTCGAG-TAATTAGCGCTCTATATACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTATATAGAGCGCTAATTACTCGAGTAATTAGCGCTCTATATACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTATATAGAGCGCTAATTACTCGAGTAATTAGCGCTCTATATACAT-3'

Other clones with same target sequence:

(none)