Construct: shRNA TRCN0000349765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148928.2-869s21c1
DNA Barcode:
GCACCGAGAAGGCTATCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf3c5 (70239)
Gene Description:
general transcription factor IIIC, polypeptide 5
Transcript:
RefSeq NM_148928.2 (NON-CURRENT)
Match location:
Position 869 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCGAGAAGGCTATCATAA
Hairpin Sequence:
5'-CCGG-GCACCGAGAAGGCTATCATAA-CTCGAG-TTATGATAGCCTTCTCGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCGAGAAGGCTATCATAACTCGAGTTATGATAGCCTTCTCGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCGAGAAGGCTATCATAACTCGAGTTATGATAGCCTTCTCGGTGC-3'

Other clones with same target sequence:

(none)