Construct: shRNA TRCN0000349781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133352.2-1551s21c1
DNA Barcode:
CACGTCCTTCTGGGCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tm9sf3 (107358)
Gene Description:
transmembrane 9 superfamily member 3
Transcript:
RefSeq NM_133352.2 (CURRENT)
Match location:
Position 1551 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGTCCTTCTGGGCATATAA
Hairpin Sequence:
5'-CCGG-CACGTCCTTCTGGGCATATAA-CTCGAG-TTATATGCCCAGAAGGACGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGTCCTTCTGGGCATATAACTCGAGTTATATGCCCAGAAGGACGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGTCCTTCTGGGCATATAACTCGAGTTATATGCCCAGAAGGACGTG-3'

Other clones with same target sequence:

(none)