Construct: shRNA TRCN0000349861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025895.3-699s21c1
DNA Barcode:
ATCAGTTTCTGTCCCTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med28 (66999)
Gene Description:
mediator complex subunit 28
Transcript:
RefSeq NM_025895.3 (NON-CURRENT)
Match location:
Position 699 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCAGTTTCTGTCCCTATTTA
Hairpin Sequence:
5'-CCGG-ATCAGTTTCTGTCCCTATTTA-CTCGAG-TAAATAGGGACAGAAACTGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCAGTTTCTGTCCCTATTTACTCGAGTAAATAGGGACAGAAACTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCAGTTTCTGTCCCTATTTACTCGAGTAAATAGGGACAGAAACTGAT-3'

Other clones with same target sequence:

(none)