Construct: shRNA TRCN0000349864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144921.1-2129s21c1
DNA Barcode:
CGGAGCAGATTGACGAGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp1a3 (232975)
Gene Description:
ATPase, Na+/K+ transporting, alpha 3 polypeptide
Transcript:
RefSeq NM_144921.1 (NON-CURRENT)
Match location:
Position 2129 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGCAGATTGACGAGATTC
Hairpin Sequence:
5'-CCGG-CGGAGCAGATTGACGAGATTC-CTCGAG-GAATCTCGTCAATCTGCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGCAGATTGACGAGATTCCTCGAGGAATCTCGTCAATCTGCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGCAGATTGACGAGATTCCTCGAGGAATCTCGTCAATCTGCTCCG-3'

Other clones with same target sequence:

(none)