Construct: shRNA TRCN0000350104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016893.4-730s21c1
DNA Barcode:
TGGTCATTTGGTTCGAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fut8 (53618)
Gene Description:
fucosyltransferase 8
Transcript:
RefSeq NM_016893.4 (NON-CURRENT)
Match location:
Position 730 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTCATTTGGTTCGAGATAA
Hairpin Sequence:
5'-CCGG-TGGTCATTTGGTTCGAGATAA-CTCGAG-TTATCTCGAACCAAATGACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTCATTTGGTTCGAGATAACTCGAGTTATCTCGAACCAAATGACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTCATTTGGTTCGAGATAACTCGAGTTATCTCGAACCAAATGACCA-3'

Other clones with same target sequence:

(none)