Construct: shRNA TRCN0000350124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033354.2-1926s21c1
DNA Barcode:
CCACAGTCAAGAGTTTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec16b (89867)
Gene Description:
SEC16 homolog B (S. cerevisiae)
Transcript:
RefSeq NM_033354.2 (NON-CURRENT)
Match location:
Position 1926 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGTCAAGAGTTTATGAA
Hairpin Sequence:
5'-CCGG-CCACAGTCAAGAGTTTATGAA-CTCGAG-TTCATAAACTCTTGACTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGTCAAGAGTTTATGAACTCGAGTTCATAAACTCTTGACTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGTCAAGAGTTTATGAACTCGAGTTCATAAACTCTTGACTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000192374