Construct: shRNA TRCN0000350249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010749.6-473s21c1
DNA Barcode:
GAGAATCAACGAGACTCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
M6pr (17113)
Gene Description:
mannose-6-phosphate receptor, cation dependent
Transcript:
RefSeq NM_010749.6 (NON-CURRENT)
Match location:
Position 473 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATCAACGAGACTCACAT
Hairpin Sequence:
5'-CCGG-GAGAATCAACGAGACTCACAT-CTCGAG-ATGTGAGTCTCGTTGATTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATCAACGAGACTCACATCTCGAGATGTGAGTCTCGTTGATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATCAACGAGACTCACATCTCGAGATGTGAGTCTCGTTGATTCTC-3'

Other clones with same target sequence:

  1. TRCN0000173503