Construct: shRNA TRCN0000350344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001260.1-971s21c1
DNA Barcode:
GCTTACCATGGACCCAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK8 (1024)
Gene Description:
cyclin dependent kinase 8
Transcript:
RefSeq NM_001260.1 (NON-CURRENT)
Match location:
Position 971 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTACCATGGACCCAATAAA
Hairpin Sequence:
5'-CCGG-GCTTACCATGGACCCAATAAA-CTCGAG-TTTATTGGGTCCATGGTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTACCATGGACCCAATAAACTCGAGTTTATTGGGTCCATGGTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTACCATGGACCCAATAAACTCGAGTTTATTGGGTCCATGGTAAGC-3'

Other clones with same target sequence:

(none)