Construct: shRNA TRCN0000350418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040260.2-1985s21c1
DNA Barcode:
AGTCAAATGCTTCAGGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCLK2 (166614)
Gene Description:
doublecortin like kinase 2
Transcript:
RefSeq NM_001040260.2 (NON-CURRENT)
Match location:
Position 1985 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCAAATGCTTCAGGTAAAT
Hairpin Sequence:
5'-CCGG-AGTCAAATGCTTCAGGTAAAT-CTCGAG-ATTTACCTGAAGCATTTGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCAAATGCTTCAGGTAAATCTCGAGATTTACCTGAAGCATTTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCAAATGCTTCAGGTAAATCTCGAGATTTACCTGAAGCATTTGACT-3'

Other clones with same target sequence:

(none)